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A strategy for rapidly making a DNA vaccine and treatment for disease caused by COVID19-Part three

George Pieczenik

Rutgers University, School of Environmental and Biological Sciences, New Brunswick, N.J. 08901, USA

E-mail : bhuvaneswari.bibleraaj@uhsm.nhs.uk

DOI: 10.15761/MCRR.1000145

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In Part 2 of our last paper on Rapidly Making a Vaccine and Treatment for the disease caused by Covid19 we defined three possible epitopic sequences [1]. These three epitopic sequences were defined by a Kimura peptide analysis we developed to determine that selection for these sequences were on the amino acid sequence and not on the nucleotide sequence. This is evidence of the importance of these amino acid sequences to the survival of this virus for either binding or a function yet to be determined. These sequences are also on the surface of the virus as they have been identified by their being amino acid sequences identical to the SARS epitopes and bindable by antibodies.

In this paper, we take the three amino acid sequences and create a DNA sequence that can code for these three with the one unique characteristic is that the codons to be used are not the codons used by the virus but are codons that are most frequently used by human codings. These most frequently used human codons were identified by GenScripts algorithm and are listed in Table 1 below:

Table 1. GenScript Codon Usage Frequency Table

Human aa

Most frequently used human codon/aa

F

TTC, TTT

L

CTG

Y

TAC

H

CAC

Q

CAG

I

ATC

M

ATG

N

AAC

K

AAG

V

GTG

D

GAC

E

GAG

S

AGC, TCC

C

TGC

TRM

TGA

W

TGG

P

CCA, CCT

R

AGG, AGA

T

ACC

A

GCC, GCA

G

GGC

G

GGC

Using these codons we can back translate the three epitopic sequences identified in Part 2 of these papers. The GenScript algorithm was used for this back translation by Dr.Z.Hu.

These sequences inserted into pcNDA3.1(+) via KpnI and NotI with a CMV promoter ahead of them creates a human DNA vaccine that will replicate its product (GenScript). The total cost of making this vector which expresses the epitopic peptide that will create both antibodies in the patient, block Covid19 from binding and compete intracellularly with Covid19 is $240 with shipping (GenScript, Dr. Z.Hu pers.comm). These DNA immunization vectors can be easily delivered as a gut microbe [2]. This is worth testing immediately.

Figure 1 shows the back translations with a terminator codon (tga) and a Kozak initiation sequence added (gccgccacc) [3].

Figure 1. DNA vaccine for corona epitopes

These DNA sequences will be transcribed and translated into an epitopic amino acid sequence which will induce antibodies against Covid19. In addition, these amino acid sequence will bind competitively and block the necessary sites for Covid19 survival. Therefore these sequences can be used directly as a DNA vaccine [4].

In addition, these DNA sequences can also be inserted using CRISPR and transposon technology [5] as a continuous source of blocking peptides and antigenic peptides. At some point, there will be a balance of antibodies formed against Covid19 and the amount of blocking peptide formed. At this point, the disease will be treated and the infectivity suppressed. These DNA sequences will compete with the Covid19 virus for sequences needed by both and will limit the ability of Covid19 to be expressed maximally without this Genotypic competition [6].

Editorial Information

Editor-in-Chief

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Short Communication

Publication history

Received date: March 09, 2020
Accepted date: March 17, 2020
Published date: March 20, 2020

Copyright

©2020 Pieczenik G. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

Pieczenik G (2020) A strategy for rapidly making a DNA vaccine and treatment for disease caused by COVID19-Part three. Med Case Rep Rev 3. DOI: 10.15761/MCRR.1000145

Corresponding author

George Pieczenik

Rutgers University, School of Environmental and Biological Sciences, New Brunswick, N.J. 08901, USA

E-mail : bhuvaneswari.bibleraaj@uhsm.nhs.uk

Figure 1. DNA vaccine for corona epitopes

Table 1. GenScript Codon Usage Frequency Table

Human aa

Most frequently used human codon/aa

F

TTC, TTT

L

CTG

Y

TAC

H

CAC

Q

CAG

I

ATC

M

ATG

N

AAC

K

AAG

V

GTG

D

GAC

E

GAG

S

AGC, TCC

C

TGC

TRM

TGA

W

TGG

P

CCA, CCT

R

AGG, AGA

T

ACC

A

GCC, GCA

G

GGC

G

GGC