Impact of environmental noise on insects’ physiology and ethology-A study on ants as models

Noise is one of the environmental factors which can impact insects’ physiology and ethology. In the present work, we examined the effects of two kinds of noise: beats (42 and 200 beats per minute) and flowing water noise (a natural environmental sound) on ants’ (used as insect model) locomotion, orientation, audacity, tactile perception, brood caring behavior, aggressiveness against nestmates and against aliens, escaping behavior, cognition and short-term memory. We found that 42 and 200 BPM increased the ants’ sinuosity of movement, decreased their orientation capability, audacity, tactile perception, brood caring behavior, escaping ability, cognition and short-term memory, and induced slight aggressiveness against nestmates. On the contrary, flowing water noise did not affect the above cited physiological and ethological traits, and even somewhat enhanced some ones. Each experiment being short lasting, the impact of the experimentally added noise very quickly vanished after the noise was stopped. Nevertheless, it can be concluded that brutal, choppy noise adversely affects ants’ (and probably other organisms’) health (at least their physiology and ethology), and should thus be avoided in their vicinity, and that smooth calming noise has a beneficial impact on them, reducing stress and improving social relationship as well as cognition and memory for instance. *Correspondence to: Marie-Claire Cammaerts, 27, square du Castel Fleuri, 1170, Bruxelles, Belgium, E-mail: mccammaerts@gmail.com


Introduction
It is well-known that numerous environmental parameters, including those created by humans, influence the organisms' physiology, behavior and general health [1]. These environmental parameters are, for instance, the food availability and consumption rates, the temperature, the humidity, the light, presence and concentrations of chemical compounds, magnetic and electrical field, presence of predators, population density, etc…. Sound is one of those factors that can influence the physiology and/or the behavior of organisms, including humans [2].
A very large number of organisms rely on sounds, i.e. on acoustic vibrations perceptible or not by humans, for performing a large variety of behaviors. For instance, such vibrations are known to be used for communicating and mating in numerous insects, fishes, amphibians, birds, and mammals [3]. As the environment baseline level of vibrations could be greatly increased in anthropogenicaly disturbed environments, there is growing concerns about the effects of those "artificial" vibrations/sounds on animals' (including insects') and plants' physiology and ethology [4][5][6][7][8][9][10][11][12]. Very recently, Barton and coauthors [13] have studied experimentally the effect of noise pollution on a model ecosystem, showing negative effects of noise pollution through trophic cascades.
In anthropogenicaly modified environment, ants play major roles as predators and can even "shape" the trophic structure of the local communities [14]. Considering how much ants relies on vibrations for performing major social behaviors [15], we can express some concerns about the potential effects of noise pollution, i.e. artificial high levels of vibrations, on ant's physiology and/or behavior, eventually leading to some cascades of negative effects.
The present study, i.e. examining the influence of two kinds of noise on ants used as insect models, has thus two aims: providing some insight on potential harmful effects of artificial sounds on ants, and exploring the idea of using ants as organism model to investigate effects of noise pollution on humans, as both organisms are sensitive to vibrations.
The two kinds of used noise were, firstly, a brutal one, beats, and secondly a smooth one, flowing water noise. The controls were the ants' physiological and ethological traits assessed without any artificial noise (only baseline noise, considered as nearly constant). Here below, before relating our methods and results, we explain why we experimented on ants, which species we used and what we know on it, and we cite the different physiological and ethological traits we examined.

Why using ants as models?
Most non-human animals' and humans' physiological and ethological traits are fundamentally similar [16]. They are often firstly examined on animals as models (e.g. fruit flies, cockroaches, bees, mice, monkeys), and then studied on humans [17]. Insects are often used as model organisms due to their rapid development and easy maintenance in laboratory [18]. Hymenoptera, among others, are used [19]. Ants could advantageously be so [20]. Indeed, these social insects present, among others, colonial regulation, labor division and information exchange thanks to tactile and chemical signals (pheromones) [15,21,22]. They construct sophisticated nests, care of their brood, and chemically mark the different parts of their habitat [21]. They navigate, recruit congeners, relocate their nest, clean its inside and create cemeteries [15]. According to such a complex biology/ behavior, they could serve as biological models for various studies. The impact of stressors or environmental changes could be examined on them, and hypothesis of effects of these stressors on other organisms can be emitted.

Which species was used?
We intensely studied the ants of the genus Myrmica, looking, among others, to their ecology, eyes morphology, angle of vision, visual perception, recruitment strategy, navigation system, learning [23], and to the ontogenesis of some of their abilities [24]. The study of the effect of electromagnetic fields (EMF) on their learning, memory capabilities and responses to pheromones showed that they could be used as biological models [25,26]. Effectively, they were so while we examined on them the potential harmful impact of products used by humans [27][28][29][30]. Effects recorded in humans were also observed in ants with good similarities in the nature of those effects, and other harmful effects, from which humans may potentially suffer, were recorded in these studies. In the present work, we used again the ant M. sabuleti Meinert 1861 as insect models for studying the consequences, on physiological and ethological traits, of added environmental noise, to investigate the effects of noise pollution on insects, and to evaluate the potential physiological and behavioral impact of noise pollution on human beings.

Which traits were examined?
We examined 11 traits on ants living under normal conditions, then on these ants living with added noise. These traits were: speed of locomotion, sinuosity of movement, orientation ability, audacity, tactile (pain) perception, brood caring, aggressiveness towards nestmates, and aggressiveness against aliens, escaping ability, cognition, and memory. The simple, rigorous experimental protocols allowing assessing these traits are already well established and have successively been used many times [27][28][29][30], what may permit comparisons with effects of other previously examined factors.

Collection and maintenance of ants
The experiments were made on two colonies of M. sabuleti collected in June 2018, in an abandoned quarry located at Marchin (Belgium). Each colony was maintained, as usual [27][28][29][30], in two glass tubes half filled with water, a cotton plug separating the ants from the water, and these nest tubes were deposited in a tray (34×23×4 cm). The ants were fed with pieces of Tenebrio molitor larvae (Linnaeus, 1758) delivered three times per week, and with sugar water permanently provided in cotton plugged tubes. The ants of the same colony are here often named 'nestmates' . The ambient temperature was ca 20°C, the humidity 80%, the lighting 330 lux, and the electromagnetism surrounding field 2 µWm 2 , all this being optimum for the species. The acoustic baseline was measured thanks to a professional sound level meter data logger (Velleman ® -DEM202). Its median (and quartiles) equaled 40.0 (39.0-41.0) decibels; its mean (and standard deviation) equaled 40.4 (1.3) decibels.

Artificial environmental parameters used
Two different beats of a metronome, 42 beats per minute (BPM) and 200 BPM, were successively used for each examined ants' trait, after the corresponding control experiment was made ( Figure 1A). In other words, for each examined trait, the control experiment was made,

Ants' physiological and ethological traits examined
Linear and angular speeds, orientation to an alarm signal: These traits were assessed on ants walking in their tray, the speeds without stimulating the ants, the orientation by stimulating them with a nestmate tied to a piece of white paper ( Figures 1B1 and 2B2). A tied nestmate emits its mandibular glands attractive pheromone. As in previous works [27][28][29][30], for the ants' speeds as well as for their orientation, the trajectory of 40 workers was recorded and analyzed thanks to appropriate software [31]. The linear speed (in mm/s) is the length of a trajectory divided by the time spent to travel it. The angular speed (in angular degree/cm=ang.deg/cm) is the sum of the angles made by successive adjacent segments, divided by the length of the trajectory. The orientation (in ang. deg.) towards a given location is the sum of the successive angles made by the direction of the trajectory and that towards the location, divided by the number of measured angles. When the value of orientation is lower than 90°, the animal has a tendency to orient itself towards the location; when it is larger than 90°, the animal has a tendency to avoid the location. The median and quartiles of each distribution of 40 values were established.
Audacity: As in previous works [27][28][29][30], a tower attached to a platform, both made of strong white paper (Steinbach ® , the tower height=4 cm; the tower diameter=1.5 cm), was deposited in the ants' tray, and those present on it were counted 10 times over 10 min (Figures 1C1, 1C2, 2C1 and 2C2). The mean and the extremes of the recorded numbers were established, and the numbers obtained for the two colonies as well as over two successive minutes were added for statistical analysis purpose.

Tactile (pain) perception:
Ants perceiving the uncomfortable character of a substrate walk on it with difficulties, slowly and sinuously. Individuals which weakly perceive this uncomfortable character, move on a rough substrate more quickly and less sinuously. So, the ants' locomotion on a rough substrate allows evaluating their tactile perception. As in previous works [27][28][29][30], a folded piece (3×(2+7+2)11 cm) of emery paper n° 280 paper was tied to the borders and the bottom of a tray (15×7×4.5 cm), dividing it into a first zone 3 cm long, a second one 3 cm long containing the emery paper, and a last one 9 cm long. Such an apparatus was built for each colony. To make an experiment, 12 ants of each colony were transferred into the first zone of their apparatus, and when they walked on the rough substrate (Figures 1D1, 1D2, 2D1 and 2D2), their linear and angular speeds were assessed as usually (see above).
Brood caring: For each colony, a few larvae and/or nymphs were removed from the nest and set in front of the entrance. Five of these  (2). A: experimental design. Red square = metronome. B: ants having soon reached a tied nestmate emitting its alarm pheromone. C: ants reacting to an unknown risky apparatus. D: ants moving on a rough substrate very well (2) but poorly (1) perceiving this uncomfortable character. E: ants in an enclosure provided with a small exit easily (1) but not (2) found. F: ants taking care of nymphs and larvae experimentally removed from the nest, correctly (1) and not correctly (2) re-entering them. G: two nesmates not at all (1) and somewhat (2) presenting aggressiveness to one another. H: ants obliged to cross a path with twists and turns for reaching a large area provided with wet cotton, six (1) and three (2) having succeeded in doing so. I: ants, conditioned to a hollow green cube, tested in a Y-apparatus provided with this cue, and giving the correct (1) and the wrong (2) response. Red circles: ants larvae or nymphs, and the ants' behavior towards them, were observed ( Figures 1F1, 1F2, 2F1 and 2F2) and the larvae or nymphs among these 5 + 5=10 observed which were still not replaced in the nest after 5 s, 2, 4, 6, 8, and 10 min were counted.
Aggressiveness against nestmates and aliens: As in previous works [27][28][29][30], these traits were assessed in the course of five dyadic encounters performed for each colony, using an observed ant and either a nestmate or an alien ant, in a cylindrical cup (diameter=2 cm, height=1.6 cm, the borders being covered with talc). Each time, the behavior of the observed ant was characterized by the number of times it did nothing (level 0 of aggressiveness), contacted the opponent with its antennae (level 1), opened its mandibles (level 2), gripped the other ant (level 3), tried to sting or stung the opponent (level 4) ( Figures 1G1, 1G2, 2G1, 2G2, 2G'1 and 2G'2). The numbers obtained for the two colonies were added. Also, as in previous works [30], the ants' aggressiveness was assessed by the variable "a"=number of aggressiveness levels 2 + 3 + 4/number of levels 0 + 1.

Escaping behavior:
As in previous works [27][28][29][30], for each colony, 6 ants were enclosed under a reversed polyacetate glass (h=8 cm, bottom diameter=7 cm, ceiling diameter=5 cm) set in the ants' tray. A notch (3 mm height, 2 mm broad) had been made in the rim of the glass bottom to allow the ants escaping ( Figures 1E1, 1E2, 2E1 and 2E2). Then, after 30 s, 2, 4, 6, 8, 10 and 12 min, the escaped ants and those still enclosed were counted. The results obtained for the two colonies were added, and the ants' ability in escaping was also quantified by the variable 'n° of ants escaped after 12 min/12' .
Cognition: This trait was assessed for the first time when studying the effects of nicotine [32]. In the present work, for each colony, two pieces of white paper (Steinbach ® , 12×4.5 cm) duly folded were again inserted in a tray (15×7×4.5 cm), dividing so this tray into a first small loggia, then a path with twists and turns, and finally a large loggia containing a piece of wet cotton ( Figures 1H1, 1H2, 2H1 and 2H2). For making an experiment, 15 ants of each colony were transferred into the first loggia of their apparatus, and those present in this loggia and in the Memory: First, under control conditions, a green hollow cube was set above the entrance of the sugar water tube of the two colonies, and the ants went so through visual conditioning. Their acquisition of such a conditioning was checked by making tests over time until their conditioning scores no longer increased. To make a test, 10 ants of colony A and of colony B were individually transferred into the entrance of a Y-apparatus provided with a green hollow cube in one of its branches. The Y-apparatus was made of strong white paper and was deposited in a tray (30×15×4 cm). The green cube was randomly located in the right or the left branch of this Y-apparatus. Moving into the branch containing the cue was considered as giving the correct response ( Figures 1I1 and 1I2). Each test provided the response of 20 ants, and from them, the proportion of correct responses. After the ants were duly conditioned, identical tests were made under the added environmental parameters (i.e. 42 BPM then 200 BPM during a first series of experiments, flowing water noise during a second series). The proportions of correct responses were again, each time, obtained.

Statistical analysis
The data about ants' linear speed, angular speed, orientation, tactile perception, aggressiveness against nestmates and against aliens, as well as memory were converted into frequencies or ranks. The statistical comparison between the control condition and that with an added environmental parameter was made using the non-parametric χ² test [33]. Concerning the ants' audacity, brood caring, escape behavior and cognition, the statistical analysis of the comparison was made using the non-parametric test of Wilcoxon [33].

Impact of beats
Locomotion: The ants' locomotion was impacted by 42 BPM as well as by 200 BPM ( Table 1). The linear speed was lower than the control one, significantly during 42 BPM (χ²=8.57, df=2, 0.01<P<0.02) and highly significantly during 200 BPM (χ²=57.84, df=2, P<0.001). The difference between the impact caused by 42 and 200 BPM was significant (χ²=52.27, df=2, P<0.01). The ants' angular speed was higher during beats than during the control, this being highly significant under 42 BPM (χ²=68.84, df=1, P<0.001) as well as under 200 BPM (χ²=76.09, df=1, P<0.001), and the difference between the impact of these two kinds of beats was significant (χ²=12.69, df=2, 0.001<P<0.01). In fact, under beats, the ants walked erratically, shortly stopped, turned, and came back on their way. Beats have thus a pronounced effect of the locomotion (so on the physiology and the behavior).
Orientation: This trait was affected by 42 BPM as well as by 200 BPM ( Figure 2B) ( Table 1). The impact was highly significant for 42 BPM (χ²=16.28, df=2, P<0.001) and for 200 BPM (χ²=24.96, df=2, P<0.001), and the difference of impact intensity between these two kinds of beats was significant (χ²=9.40, df=2, 0.001<P<0.01). Such an impact was due to the ants' change of locomotion (see above), as well as to the ants' larger attention to (stimulation by) the beats than to (by) the alarm signal emitted by the tied nestmate.  (Table 1). Indeed, the ants' linear speed on a rough substrate was larger than the control one, this being not significant for 42 BPM (χ²=4.27, df=2, 0.10<P<0.20), and significant for 200 BPM (χ²=10.54, df=2, 0.001<P<0.01). Also, the ants' sinuosity was lower than the control one, and this was significant for 42 BPM (χ²=25.33, df=2, P<0.001) as well as for 200 BPM (χ²=28.80, df=1, P<0.001). The difference of the impact on the ants' tactile perception caused by the two kinds of beats was slightly significant (concerning the linear speed: χ²=3.02, df=1, 0.05 ~ P<0.10; concerning the sinuosity: χ²=6.01, df=2, 0.02<P<0.05). Beats decreased thus the ants' perception what was in agreement with the results obtained when examining the impact on ants' orientation and audacity (see above). Similarly to what was then observed, in the present experiment, the ants seemed to be more affected by the noise than by the uncomfortable character of the substrate.
Brood caring: Under beats, ants less well re-entered the larvae and nymphs experimentally removed from the nest than when they had to do so without any noise ( Figures 1F1 and 1F2) ( Table 1). Such a result was significant for 42 BPM as well as for 200 BPM (N=5, T=+15, P=0.031). A slight difference between the ants' behavior under the two kinds of beats could be observed, but was not significant (N=3, NS). In agreement with previous (here above related) observations, the ants seemed to be too perturbed by the beats for efficiently reacting in front of brood removed from the nest.

Aggressiveness against nestmates:
This ethological trait was significantly affected by beats, i.e. ants presented some slight aggressiveness towards nestmates ( Figures 1G1 and 1G2)  Escaping behavior: This ethological trait was affected by beats, and was more affected by 200 BPM than by 42 BPM (Figures 1E1 and 1E2) ( Table 1). Indeed, under beats, the ants essentially moved erratically all around the entire area of the enclosure, and not exclusively along the rim provided with the exit, this being more pronounced under 200 than under 42 BPM. The ants thus only seldom found the exit, and the difference of ants escaped (or still enclosed) was significant between the control and 42 BPM, the control and 200 BPM, as well as between 42 and 200 BPM (each time, N=6, T=-21 (+21), P=0.016). The present experiment allowed assessing the enclosed individuals' state of stress and some of their cognition. At this time, we can conclude that beats increased the ants' state of stress, and two following experiments examined if such a noise also affects their cognitive capabilities (see below).
Cognition: This physiological trait was affected by 42 BPM and by 200 BPM (Figures 1H1 and 1H2) ( Table 1). The numbers of ants still present in the small area and of those having reached the large area statistically differed between the control and 42 BPM (N=7, T=28, P=0.008; N=4, T=-10, P=0.063 respectively), between the control and Traits
Orientation: Under flowing water noise, the ants perfectly oriented themselves towards a tied nestmates (Figures 2B2) ( Table 2). They did so "calmly", and their orientation capability did not differ from the control one (χ²=0.62, df=2, 0.70<P<0.80). The "calming" effect of flowing water did not reduce the ants' usual reaction in the presence of their species' alarm signal.
Audacity: Though being calmer, under flowing water noise, the ants went on coming, to some extent, onto an unknown apparatus ( Figures 2C1 and 2C2). There was no statistical difference between the control and the flowing water noise situations as for the numbers of ants counted on such an apparatus (Table 2) (N=4, T=-3, +7, P=0.313).
Brood caring: Under flowing water noise, the ants re-entered the larvae and the nymphs experimentally removed from the nest exactly as they did in the absence of noise ( Figures 2F1 and 2F2) (Table 2). There was no difference at all, as for this social behavior, between ants tested under the two kinds of situation (N=0, NS). Consequently, flowing water noise did not affect the individuals' social relationship, a deduction checked again thanks to the following experiment (see below).
Aggressiveness against nestmates: Unexpectedly, ants tested under flowing water noise presented still less aggressiveness towards nestmates than ants tested without noise ( Figures 2G1 and 2G2) (Table  2). This difference between ants observed under the two kinds of situation was statistically significant (χ²=9.98, df=2, 0.001<P<0.01). In fact, under flowing water noise, ants more often stayed doing nothing (65) and made less antennal contacts (71) (65/71=0.92) than ants living without that noise (27/65=0.42). As a matter of fact, under flowing water noise, the ants more often calmly stayed aside their nestmates. This noise ameliorated thus somewhat the individuals' social relationship (a deduction in agreement with that of the previous experiment: see above).

Aggressiveness against aliens:
Flowing water noise did not impact the ants' aggressiveness against aliens (Figures 2G'1 and 2G'2) ( Table  2): the ants went on attacking an alien just like went living under no noise situation. The numbers of levels 0 to 4 behaviors were statistically similar for ants living under no and flowing water noise (χ²=2.54, df=3, 0.30<P<0.50). Consequently, even if being somewhat "calmer", the ants perceiving flowing water noise adequately reacted in the presence of an alien, i.e. a danger, what was in agreement with our results on ants' orientation towards an alarm signal (see above).
Escaping behavior: Ants experimented under flowing water noise moved more slowly in the enclosure, essentially along its rim, and thus more quickly found the exit than ants living without noise (Table 2) (Figures 2E1 and 2E2). This difference of behavior between ants tested under the two kinds of situation was significant (N=6, T=±21, P=0.016). The experiment entitled 'escape behavior' is devoted to the assessment Last area (n°) 5 4 6 Short term memory 95% 95% 90% Table 2. Impact of flowing water noise on eleven ethological and/or physiological traits. Control 1 and control 2 slightly differed due to the demographic evolution of the ant population. Ants' responses under flowing water noise (column 4) were compared to ants' responses during control 2 (column 3). Under flowing water noise, the ants moved slower (more calmly), still less aggressed their nestmates, and could better escape from an enclosure. All the cognitive abilities, ethological and physiological examined traits were thus either unchanged or improved of the individuals' state of stress. The more stressed are the individuals, the less promptly they could find the exit and escape. It could thus be concluded that flowing water noise reduced the ants' state of stress, as did for instance an extract of four plants, Sedinal plus® [34].
Cognition: Under flowing water noise, the ants seemed calmer and better progressed through the twists and turns path than ants tested in the absence of noise ( Figures 2H1 and 2H2) ( Table 2). The difference of behavior, during the present experiment, i.e. of cognitive abilities, between ants tested under the two kinds of noise situation (flowing water or no noise) was significant (small area: N=7, T=-28, P=0.008; large area: N=3, NS due to the small size of the sample). Note also that cognitive abilities are generally better when the individuals' state of stress is rather low, and this was the case under flowing water noise (see above).

Short term memory:
Under flowing water noise, the ants' response to the visual cue (and thus their short-term memory) was identical to that of ants living under baseline noise level ( Table 2). The difference of ants' response between the two kinds of noise situation was not at all significant (χ²=0). Consequently, flowing water noise may have a calming effect (see above) but without affecting the individuals' memorizing capability.

Discussion and conclusion
The slight differences observed between the first and the second controls are most likely due to the demographic evolution of the ant population. The callows emerged in spring progressively became to forage and were thus experimented together with the older foragers. This points out the importance of making controls before any test experiment, a precaution we always took in the course of our studies [30,34].
The analysis of the added environmental noises (i.e. beats and flowing water noise) and the results showed that the nature as well as the intensity of such noises accounted for their impact on the ants' physiology and ethology: flowing water noise had beneficial effects, beats had harmful ones; 200 BPM had stronger harmful effects than 42 BPM.
We did not examine the impact of beats and of flowing water noise on the ants' long-term memory because this would have lasted for a longer time period (a few days to a few weeks). For such longer-term studies, we should have used new colonies, and should have taken into account the potential confounding factor of the demographic evolution of the colonies as well as the potential harmful cumulative effects of beats noise. Waste of large amounts of water could also be a concern in the case of a longer study. Considering our results, ants seems thus somewhat influenced by artificially produced beats and by environmental water noise, the former seeming to produce negative effects on ants' behaviors and the later to produce beneficial ones. Let us recall that ants play an important role in a large variety of ecosystem [14]. Moreover, ants are largely present in environments transformed by humans, like cities or industrial landscapes and can thus be exposed to artificial sounds and vibrations of high intensities. Therefore, it is plausible that artificially produced sounds (or more generally speaking, vibrations) could generate harmful effects on ant's colonies activities, and maybe causing trophic cascades as illustrated by Barton and coauthors [13].
Of course, insects other than ants can be influenced by noise. Let us cite the works compiled by Hedwig [35], the recent experimental study of Brandon et al. [13] (and references therein), Lee et al. [36] and Costello et al. [6], among others. Living organisms other than insects can also be impacted by environmental noise. Numerous examples exist: e.g. plants [7], fishes [37], birds [5], and marine mammals [38] among others. Dogs and cats can nowadays be calmed thanks to recorded natural calming noise [39]. Such an effect of music is also valid for other mammals, i.e. for rats [40]. Sounds can also have very harmful effects on vertebrates [41,42] and are used for pest controls in crop management [43,44].
Concerning the humans, they are evidently also affected by environmental noise, frequently while they are performing activities [42,[45][46][47][48]. Of course, children are also affected by harmful noise [49]. Furthermore, sounds are increasingly used as militarized weapons [50,51] or deterrent devices [52]. Reducing noise pollution, and even using music therapy, is known to be beneficial to the humans' health [53,54]. Some kind of noise is nowadays used for avoiding that adolescents and children stay too long time in shops [55].
In conclusion, using ants as models, we found that sounds could affect individuals' physiology and ethology (e.g. the sensitive perception, stress state, social relationship, cognition, and memory), brutal noise doing so adversely, smooth noise doing so beneficially. Such a finding is in accordance with many researchers' results, these researchers working on plants, insects, fishes, birds, mammals or humans. We pledge for more researches on the potential adverse effects of artificially produced high levels of noises/vibrations on the insects communities in urban environment. Ants could be valuable model organisms for such an investigation.
As artificial sound is also well-know to produce harmful effects on peoples, we can thus advise, for humans including the children, to avoid or at least minimize the exposition to brutal, perturbing noise, and to add, as possible or necessary, some natural or manmade smooth, calming noise in the individuals' environment.
Other environmental factors can also influence the individuals' physiology and ethology (see the introduction section, [56]). In two future works, we shall examine the impact of odors and of continuous lighting exposure.

Conflicts of interest
We affirm having no conflict of interest as for the use of any noise for any animals' or humans' therapy. We work only on ants and receive no money for making our research.